Villain | Alias | Expansion | Difficulty | Actions |
|---|---|---|---|---|
| Doctor Octopus | Otto Octavius | Marvel United: Enter the Spider-Verse | ||
| Juggernaut | Cain Marko | Marvel United: X-Men | ||
| Kang the Conqueror | Marvel United: Multiverse | |||
| Kingpin | Wilson Fisk | Marvel United: Enter the Spider-Verse | ||
| Magneto | Marvel United: X-Men | |||
| MODOK | Marvel United | |||
| Molecule Man | Marvel United: Multiverse | |||
| Mystique | Marvel United: X-Men | |||
| Phoenix (Dark) | Jean Grey | Marvel United: X-Men | ||
| Prowler | Marvel United: Enter the Spider-Verse | |||
| Red Skull | Marvel United | |||
| Sabretooth | Victor Creed | Marvel United: X-Men | ||
| Taskmaster | Marvel United | |||
| Ultron | Marvel United | |||
| Venom | Marvel United | |||
| Zemo | Baron Zemo | Marvel United |